Kinetic and Equilibrium Studies on D-Glucose-D-Fructose Isomerization Catalyzed by Glucose Isomerase from Streptomyces sp.
نویسندگان
چکیده
منابع مشابه
Determination of Kinetic Parameters for Catalytic Isomerization of Glucose to Fructose by immobilized Glucose Isomerase in an Aqueous-Ethanol Medium
Catalytic isomerization of glucose to fructose by immobilized glucose isomerase in an aqueous-ethanol medium was studied. Using Michaelis-Menten equation and Haldane relationship, the main kinetic parameters were determined: Kmf, Vmf, Kmr, Vmr. By comparing the aqueous-ethanol medium with an aqueous solution, the measured values of Kmr and Kmf were increased in the aqueous-ethanol solution, whi...
متن کاملDetermination of Kinetic Parameters for Catalytic Isomerization of Glucose to Fructose by immobilized Glucose Isomerase in an Aqueous-Ethanol Medium
Catalytic isomerization of glucose to fructose by immobilized glucose isomerase in an aqueous-ethanol medium was studied. Using Michaelis-Menten equation and Haldane relationship, the main kinetic parameters were determined: Kmf, Vmf, Kmr, Vmr. By comparing the aqueous-ethanol medium with an aqueous solution, the measured values of Kmr and Kmf were increased in the aqueous-ethanol solution, whi...
متن کاملMechanism of D-fructose isomerization by Arthrobacter D-xylose isomerase.
The mechanism of D-fructose isomerization by Arthrobacter D-xylose isomerase suggested from X-ray-crystallographic studies was tested by detailed kinetic analysis of the enzyme with various metal ions at different pH values and temperatures. At D-fructose concentrations used in commercial processes Mg2+ is the best activator with an apparent dissociation constant of 63 microM; Co2+ and Mn2+ bin...
متن کاملdetermination of kinetic parameters for catalytic isomerization of glucose to fructose by immobilized glucose isomerase in an aqueous-ethanol medium
catalytic isomerization of glucose to fructose by immobilized glucose isomerase in an aqueous-ethanol medium was studied. using michaelis-menten equation and haldane relationship, the main kinetic parameters were determined: kmf, vmf, kmr, vmr. by comparing the aqueous-ethanol medium with an aqueous solution, the measured values of kmr and kmf were increased in the aqueous-ethanol solution, whi...
متن کاملSucrose/D-Glucose/ D-Fructose
1. Bottle 1 with approx. 0.5 g lyophilizate, consisting of: citrate buffer, pH approx. 4.6; β-fructosidase, approx. 720 U 2. Bottle 2 with approx. 7.2 g powder mixture, consisting of: triethanolamine buffer, pH approx. 7.6; NADP, approx. 110 mg; ATP, approx. 260 mg; magnesium sulfate 3. Bottle 3 with approx. 1.1 ml suspension, consisting of: hexokinase, approx. 320 U; glucose-6-phosphate dehydr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1967
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.31.309